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Evaluation of babassu meal in feed for layer hens during the growth phase Rev. Bras. Ciênc. Avic.
Carneiro,MIF; Sakomura,NK; Malheiros,EB; Araújo,JA; Silva,EP da; Kawauchi,IM; Fernandes,JBK; Sousa,JRST de.
Studies were conducted to evaluate the nutritional value and inclusion levels of babassu meal (BM) in the diet of grower layer pullets in substitution to wheat meal. Digestibility, metabolism and growth trials were conducted. Twelve cecectomized roosters were used in the digestibility assay to determine the coefficients of standardized digestibility of amino acids (CSDAA). The metabolism trial was conducted with 30 adult roosters to determine the apparent metabolizable energy corrected for nitrogen (AMEn) of BM. A growth trial was performed to determine replacement levels of wheat midds by BM diet using 360 six-week-old commercial layer pullets. BM was included at the 0, 75 and 150 g/kg of BM, during grower and development rearing phases, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternative feedstuffs; Nutritional composition; Digestibility; Performance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200011
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Evaluation of corn cultivars harvested at two cutting heights for ensilage R. Bras. Zootec.
Caetano,Hamilton; Oliveira,Mauro Dal Secco de; Freitas Júnior,José Esler de; Rêgo,Aníbal Coutinho do; Rennó,Francisco Palma; Carvalho,Marina Vieira de.
The objective of this study was to evaluate the agronomic characteristics, bromatological-chemical composition and digestibility of 11 corn cultivars (Zea mays) harvested at two cutting heights. Cultivars D 766, D 657, D 1000, P 3021, P 3041, C 805, C 333, AG 5011, FO 01, CO 9621 and BR 205 were evaluated when they were harvested 5 cm above ground (low) and 5 cm below the insertion of the first ear (high). The experiment was designed as random blocks, with three replicates, arranged in an 11 x 2 factorial scheme. Cultivars presented similar productions of forage dry matter and grains. Percentages of stalk, leaf, straw, cob and kernel fractions were different among cultivars, as well as dry matter content of the whole plant at harvest. Considering the whole...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Neutral detergent fiber; Plant fractions; Productivity; Plant fractions; Water soluble carbohydrates.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000100003
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Evaluation of different corn textures in dry grain or silage forms for piglets from 7 to 15 kg R. Bras. Zootec.
Vieira Neto,José; Cantarelli,Vinícius de Souza; Fialho,Elias Tadeu; Zangeronimo,Márcio Gilberto; Amaral,Nikolas de Oliveira; Rodrigues,Paulo Borges.
The objective of this study was to evaluate different textures and storage forms of corn in piglet diets. Two experiments were conducted, one of which tested digestibility of nutrients and the other diet performance. In the digestibility trial, 24 crossbred (Landrace X Large White) barrows with an initial weight of 18.7 ± 1.5 kg were used. They were kept in metabolic cages in a randomized block design using the factorial arrangement 2 X 2 (type of corn - dent and flint X type of storage - moist grain silage and dry grain) and six replicates, with one animal as the experimental unit. The values of metabolizable energy (ME) obtained were 3841 kcal of ME/kg of dry matter (DM) from dry corn, 3912 kcal of ME/kg of DM from moist corn, 4022 kcal of DM from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Ensilage; Nursery phase; Nutrition; Performance; Swine.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000900017
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Evaluation of milled pearl millet in the feeding of growing rabbits R. Bras. Zootec.
Catelan,Fernanda; Scapinello,Claudio; Figueira,Josianny Limeira; Oliveira,Andréia Fróes Galuci; Motta,Ana Carolina Monteiro.
Two experiments were carried out in order to estimate the nutritional value and the performance of growing rabbits fed different levels of pearl millet (ADR 7010). In the digestibility trial, nutritional values of pearl millet were determined in 22 45-day-old New Zealand White rabbits, allotted in a completely randomized design, subjected to two treatments - a reference diet and a test diet with 70% of its volume composed of reference diet and 30% of pearl millet - and 11 replications. The apparent digestibility values of dry matter, crude protein, neutral detergent fiber and gross energy of the pearl millet were 88.7, 85.4, 24.4 and 75.0%, respectively. In the performance trial, 120 32-day-old New Zealand White rabbits were used. Rabbits were allotted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcass; Digestibility; Energy value; Performance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000800009
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Feather and blood meal in pre-starter and starter diets for broilers R. Bras. Zootec.
Xavier,Suzany Aparecida Gomes; Stringhini,José Henrique; Brito,Alexandre Barbosa de; Andrade,Maria Auxiliadora; Café,Marcos Barcellos; Leandro,Nadja Susana Mogyca.
Two experiments were carried out (pre-starter and starter phases) to evaluate the effect of feather and blood meal on performance, organ development and digestibility and retention of nutrients in broilers. In the first experiment, it was used 280 birds and in experiment 2, it was used 240 birds. The experimental diets were formulated with four levels of feather and blood meal (0%, 2%, 4% and 6%) all of them isonutritive and isoenergetic. A metabolic assay was developed on the 4th and 7th days of age in experiment 1 and on the 14th and 17th days of age in experiment 2. In these periods, one bird per experimental unit was sacrificed for determination of morphometry of the digestive organs. In experiment 1, in which it was evaluated the pre-starter phase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal origin meal; Diets; Digestibility; Digestive development; Performance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800018
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Feeding behavior and productive performance of steers fed pearl millet grain-based diets containing proportions of babassu mesocarp bran R. Bras. Zootec.
Alencar,Wanderson Martins; Restle,João; Missio,Regis Luis; Neiva,José Neuman Miranda; Miotto,Fabrícia Rocha Chaves; Freitas,Ithálo Barros de.
ABSTRACT The objective of this study was to evaluate the feeding behavior and feedlot productive performance of dairy-origin steers fed for 84 days ground pearl millet grain-based diets with 0, 120, 240, 360, and 480 g kg-1 of babassu mesocarp bran (BMB) and a standard diet based on ground corn. Thirty Holstein-Zebu steers with average initial body weight of 371.02±27 kg were used. The experimental design was completely randomized, with five replications. Dry matter intake showed better fit with the quadratic regression equation with the inclusion of BMB, reaching a maximum value in diets with 360 g of this by-product. There was no difference for dry matter intake between pearl millet- and corn-based diets. There was no difference in total digestible...
Tipo: Info:eu-repo/semantics/article Palavras-chave: By-products; Dairy-origin; Digestibility; Dry matter intake; Weight gain.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001200425
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Fine mesquite pod meal on performance, palatability and feed preference in lambs Animal Sciences
Aguiar, Luzyanne Varjão; Pedreira, Márcio dos Santos; Silva, Herymá Giovane Oliveira; Caires, Dirlane Novais; Silva, Abias Santos; Silva, Lázaro Costa da.
We evaluated the effect of inclusion of fine mesquite pod meal (FPM) in the diet on performance, preference and palatability of diets in feedlot lambs. Five diets were formulated with increasing levels of FPM (0, 1.5, 3.0, 4.5 and 6.0%). For performance evaluation, the design was completely randomized with five treatments and seven replications. For palatability and preference analysis, the same design was used, but the treatments consisted of diets with 0 and 3% FPM and fourteen replications. The palatability was assessed considering the amount of feed consumed within 30 min., and the preference, considering the feed intake after 24 hours. FPM had no influence on weight gain, but caused a reduction in intake and an increase in dry matter digestibility for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zootecnia additive; Food; Intake; Digestibility; Average daily gain; Sheep..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27804
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Fish protein hydrolysate in diets for Nile tilapia post-larvae PAB
Silva,Thiberio Carvalho da; Rocha,Joana D’Arc Mauricio; Moreira,Pedro; Signor,Altevir; Boscolo,Wilson Rogerio.
Abstract: The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cetengraulis edentulus; Oreochromis niloticus; Digestibility; Fish meal; Nutrition; Peptides; Pisciculture..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000700485
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Flemingia macrophylla in goat feeding R. Bras. Zootec.
Oiticica,Isabel das Neves; Fonseca,Carlos Elysio Moreira da; Souza,Vinícius Carneiro de; Silva,Aline Barros da; Lopes,Fernando César Ferraz; Morenz,Mirton José Frota.
ABSTRACTThe objective of this work was to evaluate the inclusion of Fabaceae Flemingia macrophylla (Willd.) Kuntze ex Merr. in the diet of lactating dairy goats arranged in a 5 × 5 Latin square. The diets were composed of 40% of concentrate and 60% of roughage, and the dietary treatments were defined by the level of Flemingia hay inclusion (0%, 8%, 16%, 24%, and 32% in the diet dry matter) replacing Cynodon dactyloncv. Tifton 85 hay. The diets were isonitrogenous, with 14% crude protein. Feed intake, nutrient digestibility, feeding behavior, and ruminal pH and ammonia nitrogen were evaluated. There was no difference in dry matter intake with the inclusion of Flemingia hay in the diet. The digestibility of dry matter, organic matter, crude protein, neutral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Feeding behavior; Leguminosae; Ruminal pH; Tropical forage.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000900335
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Forage intake and botanical composition of feed for cattle fed Brachiaria/legume mixtures Scientia Agricola
Macedo,Robert; Tarré,Ricardo Martinez; Ferreira,Elvino; Rezende,Claudia de Paula; Pereira,José Marques; Cadisch,Georg; Rouws,Janaina Ribeiro Costa; Alves,Bruno José Rodrigues; Urquiaga,Segundo; Boddey,Robert Michael.
A key contribution to study the cycling of nutrients in soil/plant/animal systems is the evaluation of the consumption of forage and their nutrients by cattle. The objective of this study was to test techniques to evaluate faecal production, in vitro digestibility, forage consumption and the proportion of legume in the acquired diet. Five Zebu steer calves were confined and fed five diets of different combinations of Brachiaria dictyoneura and Desmodium ovalifolium. All quantities of faeces were collected per animal and these values were found to compare favourably with those derived from using the chromium oxide technique. In vitro digestibility ranged from 7 to 10% higher than the actual in vivo digestibility. Faecal samples from steers fed with diets...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria dictyoneura; Desmodium ovalifolium; 13C abundance; Digestibility; Forage intake.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400002
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Forage potential of Urochloa genotypes by using leaf anatomy Ciência Rural
Mauri,Janaína; Pereira,Daniele Laís; Silva,Gabrielle Avelar; Davide,Lisete Chamma; Techio,Vânia Helena; Souza Sobrinho,Fausto; Pereira,Fabricio José.
ABSTRACT: The digestibility potential of leaves from forages depends on the amount of nutrition in their tissues, with low lignin deposition in the cell wall, mainly parenchyma and phloem. This research evaluated the leaf structure of different Urochloa genotypes and discussed its potential for evaluating digestibility. The cultivars U. brizantha, U. decumbens, U. ruziziensis and three clones of U. ruziziensis (1, 95 and 97), which are under development in breeding programs, were evaluated. Plants were grown under the recommended culture conditions for the Urochloa species. Plants were cut 60 days after sowing, and leaves were collected at 15 days of regrowth. Leaves were fixed in FAA 70 and further stored in 70% ethanol until being submitted to the usual...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Forage production; Leaf anatomy; Sclerenchyma; Lignin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400401
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Forage yield and nutritive value of Elephant grass, Italian ryegrass and spontaneous growing species mixed with forage peanut or red clover Ciência Rural
Diehl,Michelle Schalemberg; Olivo,Clair Jorge; Agnolin,Carlos Alberto; Junior,Ricardo Lima de Azevedo; Bratz,Vinícius Felipe; Santos,Juliano Costa dos.
The objective of this research was to evaluate of three grazing systems (GS) with elephant grass (EG), Italian ryegrass (IR) + spontaneous growing species (SGS); EG + IR + SGS + forage peanut (FP); and EG + IR + SGS + red clover (RC), during the winter and summer periods in rotational grazing with dairy cattle. Experimental design was completely randomized with three treatments, two replicates with repeated measures. Lactating Holstein cows receiving 1% BW-daily feed supplement with concentrate were used in the evaluation. Eight grazing cycles were performed during the experimental period. The values of pre forage mass and stocking rate were 2.52, 2.60 and 2.99 t ha-1 and 2.64, 2.77 and 3.14 animal unit ha-1, respectively for GS. Samples of forage were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis pintoi; Digestibility; Crude protein; Neutral detergent fiber; Pennisetum purpureum; Trifolium pratense..
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001845
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Hay Tifton 85 grass under nitrogen doses in different days of regrowth Animal Sciences
Andrade, Weudes Rodrigues; Sales, Eleuza Clarete Junqueira de; Monção, Flávio Pinto; Gomes, Virgílio Mesquita; Rufino, Leidy Darmony de Almeida; Pires, Daniel Ananias de Assis.
The objective of this study was to evaluate the yield, nutritional value and potential ruminal degradation of Tifton 85 hay (Cynodon spp.) harvested at four regrowth ages (28, 35, 42 and 49 days) and fertilized with two nitrogen doses (N; 100 and 300 kg N ha-1). The experimental design was a randomized block design, arranged in a 4 x 2 factorial scheme, with six replications each. Nitrogen fertilization increased (p < 0.05) dry matter yield and digestible dry matter production of hay and also lignin and crude protein contents. Nitrogen fertilization increased (p < 0.01) the dry matter yield and the digestible dry matter production of hay, as well as lignin (p = 0.01) and crude protein (p = 0.01). The neutral detergent fiber corrected for ash and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Agrárias; Zootecnia; Forragicultura e Pastagens fertilizing; Cynodon spp.; Digestibility; Neutral detergent fiber; Lignin..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37692
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Heat-resistant bacterial phytase in broiler pelleted diets Rev. Bras. Ciênc. Avic.
Carlos,TC de F; Barbosa,LCGS; Shiroma,NN; Dari,RL; Baolin,G; Yongcheng,W; Araújo,CS da S; Araújo,LF.
The objective of the study was to evaluate the effects of a heat-resistant bacterial phytase added to pelleted diets on mineral digestibility, live performance, carcass traits, and bone quality of broilers. Three treatments were evaluated: Positive control; negative control, with 0.10 points reduction in calcium level and 0.15 points reduction in available phosphorus level; and negative control + phytase at 500 FTU/kg. Mineral digestibility and bone quality results demonstrated that the evaluated phytase resisted pelleting as it increased the utilization of the minerals present in the diet.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Performance; Digestibility; Enzyme; Phytate; Phosphorus; Broiler nutrition.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000100045
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Impact of incorporating Moringaoleifera seed cake as protein source in growing lambs ration. CIGR Journal
El-Naggar, Soad Ibrahim.
Thirty growing Rahmani lambs (27.25 kg ± 0.25 kg average body weight 6-7 months old) were used to evaluate the impact of incorporating Moringaoleifera seed cake as protein source in a total mixed ration (TMR) for 90 days feeding trial followed by 21 day digestion. Moringaoleifera seed cake (MSC) was used for partial substitution of soybean meal protein by (0, 25 and 50 %) in three experimental rations, control (R1), R2 and R3, respectively. Results indicated that the feeding lambs on R3 recorded a significant (P<0.05) increases in all of nutrients digestibility and nutritive value compared with R1 or R2. All of rumen parameters, pH, ammonia and total volatile fatty acids, were in normal ranges as an indication of healthy.  Also, the highest average...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Department of animal production; National Research Centre Moringa cake; Lambs; Digestibility; Growth.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4586
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Improving grassland profitability in the Mid-continental USA by breeding for improved forage digestibility: lessons learned and applications to South American grasslands R. Bras. Zootec.
Vogel,Kenneth Paul; Miranda,Cesar Heraclides Behling.
In the early 1970's research was initiated by the cooperative USDA-ARS and University of Nebraska grass breeding program to develop perennial grasses adapted to the mid-continental USA with improved forage quality. The initial breeding and animal evaluation work focused on switchgrass (Panicum virgatum) but has since expanded to several other warm-and cool-season perennial grasses. The in vitro dry matter digestibility (IVDMD) test was selected as the measure of forage quality that was used in the breeding work because of its previous successful application on improving bermudagrass (Cynodon dactylon). In all of the grass species that we have studied to date, there is genetic variability for IVDMD and forage yield. IVDMD is a heritable trait with narrow...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Economics; Forage; Grasses; Pasture; Quality.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300017
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Improving Nutrition Utilization and Meat Quality of Broiler Chickens Through Solid-State Fermentation of Agricultural By-Products by Aureobasidium Pullulans Rev. Bras. Ciênc. Avic.
Lee,MT; Lai,LP; Lin,WC; Ciou,JY; Chang,SC; Yu,B; Lee,TT.
ABSTRACT A 35 d trial was conducted to evaluate the effects of dietary supplementation of co-fermented agricultural by-products, soybean hulls and Pleurotus eryngii stalk residue (PESR), with Aureobasidium pullulans on nutrients digestibility and meat quality in broilers fed on conventional corn-soybean meal basal diet. A total of 400 1-d-old Ross broilers were allotted to 4 dietary treatments with 4 replicate pens (25 birds per pen). Birds were fed the corn-soybean meal diets supplemented with 0% (CON), 0.5% fermented soybean hulls (0.5% FSBH), 0.5% fermented soybean hulls partially replaced with PESR (0.5% FSHP) and 1.0% FSHP. The broilers fed on the diet that contained fermented products had higher total tract apparent digestibility for hemicellulose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mushroom stalk residue; Soybean hull; Aureobasidium pullulans; Digestibility; Meat water holding capacity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400645
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In situ degradability of corn stover and elephant-grass harvested at four stages of maturity Scientia Agricola
Silva,Luis Felipe Prada e; Cassoli,Laerte Dagher; Roma Júnior,Luiz Carlos; Rodrigues,Ana Carolina de Oliveira; Machado,Paulo Fernando.
Among tropical forages, corn silage is largely used by farmers trying to explore the maximum genetic potential from the animals. However, other tropical forages, such as elephant-grass (Pennisetum purpureum), are more productive and therefore cheaper to use than corn silage. Our objective was to compare the in situ degradability of elephant-grass with that from corn hybrids, all harvested at four stages of maturity. The experimental design followed a randomized block design with nested subplots. Two corn hybrids: AG5011, ZN8392 were harvested with 25, 30, 35, and 40% dry matter (DM) in the whole plant, and separated in stem + leaf sheath + leaf blade (stover), and cobs. Elephant-grass was harvested with 30, 40, 50 and 60 days after a leveling cut. Dried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Corn silage; Digestibility; Rumen; Tropical grass.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600005
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In situ dry matter and fiber fraction degradability of the Mineirão stylos - doi: 10.4025/actascianimsci.v34i2.13138 Animal Sciences
Corrêa, Daniel Staciarini; Pontifícia Universidade Católica de Goiás; Magalhães, Roberto Toledo de; Pontifícia Universidade Católica de Goiás; Siqueira, Domingas Cruvinel Batista de; Pontifícia Universidade Católica de Goiás.
This research evaluated, using the in situ technique, the dry matter and fiber fraction degradability of the Mineirão stylo (Stylozanthes guianensis). The nylon bag method was used for the degradability assay, with two incubations in rumen-cannulated adult female cows. In each incubation, 25 samples of 7.0 g each were used. The samples were collected at 0, 6, 9, 12, 24, 36, 48, 72 and 96 hours. The experiment was conducted according to a randomized blocks design and data were submitted to analysis of variance. The maximum effective degradability was obtained in the 2% passage rate. For the 5% passage rate the Mineirão stylo showed effective degradation of 75.70% for dry matter, 59.01% for neutral detergent fiber and 76.81% for acid detergent fiber. The...
Tipo: Fieldwork; laboratory analysis Palavras-chave: Bovinocultura; Leguminosas; Degradabilidade ruminal; Cerrado cattle breeding; Leguminous species; Digestibility; Forage.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/13138
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Inclusion of exogenous enzymes to feathers during processing on the digestible energy content of feather meal for adult dogs R. Bras. Zootec.
Pacheco,Gabriel Faria Estivallet; Pezzali,Júlia Guazzelli; Kessler,Alexandre de Mello; Trevizan,Luciano.
ABSTRACT This study was conducted to determine the coefficient of total tract apparent digestibility (CTTAD) of nutrients and gross energy (GE), metabolizable energy (ME), coefficient of metabolizability of gross energy (CM of GE), and fecal characteristics of dogs fed diets with two levels of feather meal, with or without addition of an enzyme blend containing lipase and protease activity. Ten adult Beagle dogs (aged between 1 and 2 years and weighing 10.2±1.4 kg) were arranged in a 5 × 3 incomplete Latin square design with five treatments and three periods. The extruded basal diet was provided in equal amounts to all dogs (220 g/d), and 7.5 and 15% of hydrolyzed feather meal without enzymes (HFM) or HFM processed with addition of the enzyme blend (HFM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: By-product; Digestibility; Lipase; Protease; Protein source.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000600288
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